Clodronate is very effective in the treatment of hypercalcemia and cancer metastases to bones. It can significantly reduce the development of new metastases, as well as to decrease the risk of bone fractures, which is very high in such cases. Besides, it may also reduce the pain associated with this disease. Clodronate liposome, also known as clodrosome, seems to be the best way of delivering this compound to tissues.
To be closed in vivo, this effective compound is delivered encapsulated within liposomes. A liposome is lipid-based tiny particle, designed to carry different compounds in form of suspension. It is lately used in many research laboratories, as highly effective way or delivering drugs to various tissues. In this case, to bones and kidneys.
Clodronate liposome suspension should be injected to targeted tissues. While manipulating this type of suspension, some measures of precaution are necessary, such as chemical resistant gloves and clothing, safety goggles and respirators. This compound can be harmful if inhaled, absorbed through skin, swallowed or if it get in contact with your eyes. It causes different irritations, especially to mucous membranes, and you should be very careful with it.
In case of oral exposure, you should wash out mouth with water and call a physician. If your eyes were in contact with this compound, you should flush them with water for at least fifteen minutes. Dermal exposure should be treated with soap and water. If inhaled, it may cause breathing problems. Exposed person should get to fresh air, and sometimes it might be necessary to use respirator or even oxygen.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When clodronate is encapsulated within liposomes, it can be delivered to bones and kidneys. Macrophages will digest those particles, but this compound will ve released and accumulated in tissues. Creating macrophage depleted tissues and organs this way allows more successful research of macrophage functional aspects.
Clodronate liposomes application can also be used for suppression of macrophage activity in different models of autoimmune diseases, in gene therapy and similar researches. Although this method is designed especially for in vivo research, it can also be successfully used for depletion of macrophages in vitro.
It is important to keep the suspension at appropriate temperature. It shouldn't be exposed to temperatures above 30 degrees of Celsius, or frozen. The ideal storage temperature is four degrees Celsius, and in this case it can be kept one month in refrigerator. For achieving desired results, the suspension should be kept outside the refrigerator until it reaches room temperature, before injecting it to animals.
It is also important to shake the suspension until it becomes homogeneous. You should shave the skin and cleanse it with alcohol, to make sure it is disinfected. You should use sterile syringe for each animal. If you use the same one, you might get uneven dosages. Follow the procedure for injecting clodronate liposome to your test animals.
To be closed in vivo, this effective compound is delivered encapsulated within liposomes. A liposome is lipid-based tiny particle, designed to carry different compounds in form of suspension. It is lately used in many research laboratories, as highly effective way or delivering drugs to various tissues. In this case, to bones and kidneys.
Clodronate liposome suspension should be injected to targeted tissues. While manipulating this type of suspension, some measures of precaution are necessary, such as chemical resistant gloves and clothing, safety goggles and respirators. This compound can be harmful if inhaled, absorbed through skin, swallowed or if it get in contact with your eyes. It causes different irritations, especially to mucous membranes, and you should be very careful with it.
In case of oral exposure, you should wash out mouth with water and call a physician. If your eyes were in contact with this compound, you should flush them with water for at least fifteen minutes. Dermal exposure should be treated with soap and water. If inhaled, it may cause breathing problems. Exposed person should get to fresh air, and sometimes it might be necessary to use respirator or even oxygen.
Suppression of macrophage function is necessary for delivering drug to tissues. Macrophages are an essential part of natural immune system, but they also participate in or control other functions. They ingest and digest different microorganisms, and that's why you need to encapsulate some compounds within liposomes, to get through this macrophage layer.
When clodronate is encapsulated within liposomes, it can be delivered to bones and kidneys. Macrophages will digest those particles, but this compound will ve released and accumulated in tissues. Creating macrophage depleted tissues and organs this way allows more successful research of macrophage functional aspects.
Clodronate liposomes application can also be used for suppression of macrophage activity in different models of autoimmune diseases, in gene therapy and similar researches. Although this method is designed especially for in vivo research, it can also be successfully used for depletion of macrophages in vitro.
It is important to keep the suspension at appropriate temperature. It shouldn't be exposed to temperatures above 30 degrees of Celsius, or frozen. The ideal storage temperature is four degrees Celsius, and in this case it can be kept one month in refrigerator. For achieving desired results, the suspension should be kept outside the refrigerator until it reaches room temperature, before injecting it to animals.
It is also important to shake the suspension until it becomes homogeneous. You should shave the skin and cleanse it with alcohol, to make sure it is disinfected. You should use sterile syringe for each animal. If you use the same one, you might get uneven dosages. Follow the procedure for injecting clodronate liposome to your test animals.
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